[Histonet] Protocol for DAPI staining on paraffin sections

Hobbs, Carl carl.hobbs at kcl.ac.uk
Wed Jun 9 08:48:51 CDT 2021


Dear Alida
my emails to your msu.montan.edu get returned to me as undeliverable
I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- HIER.
Both stain nuclei very well.
The HIER-treated section gave a brighter nuclear positivity ( so needed to lower exposure time on taking images)
NSD though
I can post on Histonet images, if you wish

carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6810


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