[Histonet] Protocol for DAPI staining on paraffin sections

[John Kiernan]

Not everyone knows that the product of the Feulgen reaction is fluorescent. If you do the method on paraffin sections, DNA in nuclear chromatin is red with ordinary illumination. If you look with a fluorescence microscope (blue excitation) it shows as a brownish-red fluorescence in the chromatin. A similar fluorescent colour is seen in conventional PAS-stained sections, in mucus, basement membranes, etc. You need a non-fluorescent mounting medium: DPX or one of the various poly(methyl methacrylate)-based media such as Entellan.
John Kiernan
   Professor Emeritus, Dept of Anatomy & Cell Biology
   University of Western Ontario, London, Canada
      https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html
   Also  Secretary, Biological Stain Commission, Inc.
      https://biologicalstaincommission.org<https://biologicalstaincommission.org/>
= = =


<https://biologicalstaincommission.org>

________________________________
From: Alida Bailleul via Histonet <histonet at lists.utsouthwestern.edu>
Sent: June 3, 2021 7:53 AM
To: Histonet at lists.utsouthwestern.edu <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Protocol for DAPI staining on paraffin sections

Dear Histonet list,

I have made some paraffin blocks of demineralized bone and cartilage. I
would like to stain paraffin slides with DAPI (or another DNA stain).
Does DAPI staining (or other fluorescent DNA stain) work well on paraffin
sections? Does anyone have a protocol they would like to share?

I am assuming that histological stains (like the Feulgen stain would work
better?). I could also try both.

Thank you in advance

All the best

Alida

--
Dr. Alida M. Bailleul
Associate Research Fellow/Associate Professor
Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy
of Sciences
www.ivpp-avianevolution.com<http://www.ivpp-avianevolution.com>
& Research Associate of Paleontology, Museum of the Rockies, Montana State
University
Google Scholar
<https://scholar.google.co.jp/citations?user=Yn4PuWMAAAAJ&hl=en> -
ResearchGate
<https://www.researchgate.net/profile/Alida_Bailleul>
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