[Histonet] Protocol for DAPI staining on paraffin sections

Alida Bailleul alida.bailleul at gmail.com
Thu Jun 3 06:53:57 CDT 2021


Dear Histonet list,

I have made some paraffin blocks of demineralized bone and cartilage. I
would like to stain paraffin slides with DAPI (or another DNA stain).
Does DAPI staining (or other fluorescent DNA stain) work well on paraffin
sections? Does anyone have a protocol they would like to share?

I am assuming that histological stains (like the Feulgen stain would work
better?). I could also try both.

Thank you in advance

All the best

Alida

-- 
Dr. Alida M. Bailleul
Associate Research Fellow/Associate Professor
Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy
of Sciences
www.ivpp-avianevolution.com
& Research Associate of Paleontology, Museum of the Rockies, Montana State
University
Google Scholar
<https://scholar.google.co.jp/citations?user=Yn4PuWMAAAAJ&hl=en> -
ResearchGate
<https://www.researchgate.net/profile/Alida_Bailleul>


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