[Histonet] Water under sections

Colleen Forster cforster at umn.edu
Mon Jan 25 12:33:16 CST 2021

I use charged slides all the time. The best way to clear the water is to
cut the slides and air dry them overnight. The next day I follow the normal
protocol, heat in 60 degree over and then on to the rest of the protocol.

They don't really need the 56 degree oven all night long.


Colleen Forster

On Mon, Jan 25, 2021 at 11:26 AM Tyrone Genade via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
> On Saturday, January 23, 2021 1:00 PM, <
> histonet-request at lists.utsouthwestern.edu> wrote:
> >     In my lab, water under the sections is unique to charged slides.
> > And you
> >     are correct, if there is water under the section when the slides
> > are heated
> >     for antigen retrieval, the boiling (or at least very hot) water
> > will damage
> >     or entirely destroy the section.
> I had this very problem and the simple solution was to bake the slides
> overnight at 56oC (melting temp of the wax I was working with). I would
> still heat on a hot plate before dewaxing. Baking at 45oC also seemed to
> work. Whether this would work for you would depend on the sensitivity of
> your epitopes to prolonged heat.
> Good luck... every now and then a section would still detach during
> antigen retrieval.
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Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155

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