[Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining?

Colleen Forster cforster at umn.edu
Thu Apr 8 12:50:01 CDT 2021


When I have this I use a small cube of tissue that is completely unrelated
to the project as my marker. You can  even ink all surfaces on this small
piece of tissue. I process something but don't block it and keep that piece
to cut the smaller cubes from.

I keep track on a "map" and then that goes with the block. It works well.

Colleen Forster

On Thu, Apr 8, 2021 at 12:30 PM Cheryl via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi everyone-
> We have a researcher who needs to put multiple samples in one block for
> quality and cost control.  There are four different samples per tissue and
> they've been processed differently but all are FFPE.
> Is there a way to put an orientation mark in the block that transfers to
> the slide - like a section-able plastic bead or wax-based dye so we can
> embed these membranes in a specific order and she can keep track of the
> order on her future slide preps?
> I recall there was a cassette with little plastic 'fingers' to orient
> shave biopsies to embed later where the plastic cut and remained on the
> slide without interfering with any staining process or coverslipping-
> something like that??
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> admin at fullstaff.orgtkgnflght@yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455

More information about the Histonet mailing list