[Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining?

Erin McCarthy erin.mccarthy at tempus.com
Thu Apr 8 13:35:06 CDT 2021

Hi Cheryl,

I have heard of people using cucumber and processing it, but I have not
actually tried it out myself! One of my R&D friends recommended it to me
when trying to DIY some tumor microarrays. You might be able to try it out
and see if it stains well - I have never actually seen it in person myself!

On Thu, Apr 8, 2021 at 12:43 PM Cheryl via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi everyone-
> We have a researcher who needs to put multiple samples in one block for
> quality and cost control.  There are four different samples per tissue and
> they've been processed differently but all are FFPE.
> Is there a way to put an orientation mark in the block that transfers to
> the slide - like a section-able plastic bead or wax-based dye so we can
> embed these membranes in a specific order and she can keep track of the
> order on her future slide preps?
> I recall there was a cassette with little plastic 'fingers' to orient
> shave biopsies to embed later where the plastic cut and remained on the
> slide without interfering with any staining process or coverslipping-
> something like that??
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> admin at fullstaff.orgtkgnflght@yahoo.com
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Erin McCarthy, HT (ASCP)
Histology Supervisor

Tempus Labs
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Chicago IL 60654
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