[Histonet] IHC DAB

[Paula Keene Pierce]

Since you are newbies, I am just putting IHC 101 out there. 
Permanent red is for alkaline phosphatase detection and DAB is for HRP.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Friday, May 1, 2020, 10:29:00 AM CDT, Kristy Castillo via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
permanent red is working just fine.  Any ideas.

Thank you!

Kristy
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet