[Histonet] IHC DAB

[Kristy Castillo]

Thanks for the tip!

On Fri, May 1, 2020 at 8:53 AM Val Tolley <vtolley25 at gmail.com> wrote:

> It’s difficult to know without your full protocol, but you can easily test
> your DAB.
>
> Take a paper towel and add a drop of your secondary/HRP.  Add a drop of
> DAB to that.  If it doesn’t immediately turn brown, your hydrogen peroxide
> has likely degraded into water.  Make a fresh batch of DAB and try again.
>
>
>
> > On May 1, 2020, at 8:28 AM, Kristy Castillo via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > Hi Histonetters,
> >
> > We are starting our IHC (fun times), we are having trouble with the DAB
> > lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
> > permanent red is working just fine.  Any ideas.
> >
> > Thank you!
> >
> > Kristy
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