[Histonet] hard Tick histology

[Mike Tighe]

Hello,

I was wondering if someone might have some expertise on how to prepare a hard-tick for sectioning in paraffin. I have been decalcifying to soften the exoskeleton but not sure if that even helps. I have used acid decalcifying but would EDTA be more appropriate? I have punctured the ticks and used vacuum for all steps (fixation, decalcification, dehydration etc....). Still crunchy on the outside and soft in the middle!

Any help would be greatly appreciated!!

Thanks!
Mike