[Histonet] hard Tick histology

Mike Tighe mtighe at trudeauinstitute.org
Thu Mar 5 15:17:54 CST 2020


I was wondering if someone might have some expertise on how to prepare a hard-tick for sectioning in paraffin. I have been decalcifying to soften the exoskeleton but not sure if that even helps. I have used acid decalcifying but would EDTA be more appropriate? I have punctured the ticks and used vacuum for all steps (fixation, decalcification, dehydration etc....). Still crunchy on the outside and soft in the middle!

Any help would be greatly appreciated!!


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