[Histonet] IHC on Pig Tissues

[Lori Sorrells]

Good afternoon-
Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues?  I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies.  I have used a variety of blockers including various sera in the diluents and blocking reagents.  All samples are FFPE.  I routinely use the DAKO EnVision+ Dual HRP system.  Thanks in advance!

Lori Sorrells
Research Associate II
Revivicor, Inc.
1700 Kraft Drive, Suite 2400
Blacksburg, VA 24060