[Histonet] IHC on Pig Tissues

[Liz Chlipala]

Lori

You can't use a dual label detection system.  We have found most mouse polymers will cross react with porcine tissue.  We know that the dako one does.  You need to use the rabbit envision for rabbit monoclonal antibodies or with mouse monoclonals you need to use a rabbit anti-mouse and then a rabbit envision detection system.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com<mailto:liz at premierlab.com>
www.premierlab.com<http://www.premierlab.com/>

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Lori Sorrells via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 29, 2020 1:35 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC on Pig Tissues

Good afternoon-
Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers including various sera in the diluents and blocking reagents. All samples are FFPE. I routinely use the DAKO EnVision+ Dual HRP system. Thanks in advance!

Lori Sorrells
Research Associate II
Revivicor, Inc.
1700 Kraft Drive, Suite 2400
Blacksburg, VA 24060

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