[Histonet] Need a procedure

[John Garratt]

http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf


The above link will help.



www.cpqa.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Thursday, January 23, 2020 12:13 PM, Muhammad Azam <ajju33 at gmail.com> wrote:

> Anybody has validated procedure for histogel
>
> Sent from my iPhone
>
> > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote:
> > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin.
> > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma.
> > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC.
> > John
> > www.cpqa.ca
> > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> >
> > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote:
> > > Hi fellow Histonetters - I'm in need of some help, please
> > > Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method
> > > Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used?
> > > Thanks in advance. Histotechs rock!
> > > Terri L. Braud, HT(ASCP)
> > > Anatomic Pathology Supervisor
> > > Laboratory
> > > Holy Redeemer Hospital
> > > 1648 Huntingdon Pike
> > > Meadowbrook, PA 19046
> > > ph: 215-938-3689
> > > fax: 215-938-3874
> > > Care, Comfort, and Heal
> > > Histonet mailing list
> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
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