[Histonet] Need a procedure
ajju33 at gmail.com
Thu Jan 23 14:13:39 CST 2020
Anybody has validated procedure for histogel
Sent from my iPhone
> On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin.
> Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma.
> The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC.
> ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
>> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>> Hi fellow Histonetters - I'm in need of some help, please
>> Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method
>> Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used?
>> Thanks in advance. Histotechs rock!
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
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