[Histonet] Need a procedure
Tony Henwood (SCHN)
tony.henwood at health.nsw.gov.au
Thu Jan 23 16:59:50 CST 2020
Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel).
Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX.
Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated.
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, 24 January 2020 8:21 AM
To: Muhammad Azam <ajju33 at gmail.com>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Need a procedure
The above link will help.
‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Thursday, January 23, 2020 12:13 PM, Muhammad Azam <ajju33 at gmail.com> wrote:
> Anybody has validated procedure for histogel
> Sent from my iPhone
> > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote:
> > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin.
> > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma.
> > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC.
> > John
> > www.cpqa.ca
> > ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote:
> > > Hi fellow Histonetters - I'm in need of some help, please
> > > Background - We currently use agar to capture our scant cell
> > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used?
> > > Thanks in advance. Histotechs rock!
> > > Terri L. Braud, HT(ASCP)
> > > Anatomic Pathology Supervisor
> > > Laboratory
> > > Holy Redeemer Hospital
> > > 1648 Huntingdon Pike
> > > Meadowbrook, PA 19046
> > > ph: 215-938-3689
> > > fax: 215-938-3874
> > > Care, Comfort, and Heal
> > > Histonet mailing list
> > > Histonet at lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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