[Histonet] Need a procedure

[Colleen Forster]

Terri,

I agree with John on the use of Histogel. I use it routinely for all the
cell blocks and tiny matrix samples that come down and I get excellent
results.

I do a lot of IHC and the results are good.

Respectfully,

Colleen



On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Terri, I suggest you use Histogel for block preparation. It works
> exceptionally well, it is good for IHC and does not have the pitfalls of
> plasma/thrombin.
> Plasma/thrombin does work well for cell blocks but you will have to
> consider an ethical and safe source for your plasma.
> The instructions for using Histogel are in the package insert though I
> have one comment. Be careful how you warm the Histogel and use a heat
> block. Do NOT use a microwave since there is a tendency to overheat the gel
> and you will end up with poor quality IHC.
>
> John
>
>
> www.cpqa.ca
>
> ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Hi fellow Histonetters - I'm in need of some help, please
> > Background - We currently use agar to capture our scant cell blocks for
> processing. I am unfamiliar with the Plasma/Thrombin method of cell block
> preparation and am interested in comparing it to our current method
> > Request - Could you please send me your procedures for this method,
> specifically where you purchase your plasma and thrombin and what species
> are used?
> > Thanks in advance. Histotechs rock!
> >
> > Terri L. Braud, HT(ASCP)
> > Anatomic Pathology Supervisor
> > Laboratory
> > Holy Redeemer Hospital
> > 1648 Huntingdon Pike
> > Meadowbrook, PA 19046
> > ph: 215-938-3689
> > fax: 215-938-3874
> > Care, Comfort, and Heal
> >
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB      612-626-1930