[Histonet] H&E trouble shooting

Liz Chlipala liz at premierlab.com
Wed Jan 15 12:49:27 CST 2020


That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
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liz at premierlab.com<mailto:liz at premierlab.com>
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From: Wang, Weixi via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 15, 2020 11:20 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H&E trouble shooting

Hi,
I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing?
You expertise is greatly appreciated!

https://imgur.com/gallery/kpLqRT9<https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwMFaQ&c=E9plUmAGv24GJC_RgV6lkg&r=ipz9InHENRrvJEfmICylkob51vH0k--_Zwg_B4oxt68&m=reSnEx9x60Sn7qDJL7RV7v4jMeXHB7sTahlobD4ajts&s=WWb8lSKEB7ZvArMrCeT-_5-oyHjQObc80P04bgUiyRo&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwMFaQ&c=E9plUmAGv24GJC_RgV6lkg&r=ipz9InHENRrvJEfmICylkob51vH0k--_Zwg_B4oxt68&m=reSnEx9x60Sn7qDJL7RV7v4jMeXHB7sTahlobD4ajts&s=WWb8lSKEB7ZvArMrCeT-_5-oyHjQObc80P04bgUiyRo&e=%3e>


Thank you,
Weixi




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