[Histonet] H&E trouble shooting
liz at premierlab.com
Wed Jan 15 12:49:27 CST 2020
That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com<mailto:liz at premierlab.com>
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From: Wang, Weixi via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 15, 2020 11:20 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H&E trouble shooting
I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing?
You expertise is greatly appreciated!
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