[Histonet] H&E trouble shooting

[Wang, Weixi]

Hi,
I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing?
You expertise is greatly appreciated!

https://imgur.com/gallery/kpLqRT9<https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwMFaQ&c=E9plUmAGv24GJC_RgV6lkg&r=ipz9InHENRrvJEfmICylkob51vH0k--_Zwg_B4oxt68&m=reSnEx9x60Sn7qDJL7RV7v4jMeXHB7sTahlobD4ajts&s=WWb8lSKEB7ZvArMrCeT-_5-oyHjQObc80P04bgUiyRo&e=>


Thank you,
Weixi




*** Important Notice About St. Josephs emails ***
St. Josephs Regional Medical Center is using Zix to encrypt any e-mails containing Protected Health Information (PHI).  If you receive an encrypted email from a person at St. Josephs the body of the message will indicate that you have a "New Zixcorp secure email from St. Josephs Regional Medical Center."  You will need to click on the link in that email to retrieve the message.  For more information please visit http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/introduction.  For help in retrieving a secure email that you’ve received from St Josephs please go to http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/receiving-encrypted-email.  

CONFIDENTIAL COMMUNICATION
THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE.