[Histonet] H&E trouble shooting

[Bert Dotson]

Excessive heat during slide drying can look like this but I think Liz has the most likely culprit. More specifically I would say there has been water carryover into your first clearing station during processing. Reagents not changed frequently enough or a low-grade mistakenly placed where 100% should be. Regards, Bert


-----Original Message-----
From: Liz Chlipala <liz at premierlab.com> 
Sent: Wednesday, January 15, 2020 1:49 PM
To: Wang, Weixi <wangweixi at sjhmc.org>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] H&E trouble shooting

That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308
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From: Wang, Weixi via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, January 15, 2020 11:20 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H&E trouble shooting

Hi,
I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing?
You expertise is greatly appreciated!

https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=8qzwuTTHy5TnitTfvT39e_57wxXJFJ2nygQXLWjNxVo&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwMFaQ&c=E9plUmAGv24GJC_RgV6lkg&r=ipz9InHENRrvJEfmICylkob51vH0k--_Zwg_B4oxt68&m=reSnEx9x60Sn7qDJL7RV7v4jMeXHB7sTahlobD4ajts&s=WWb8lSKEB7ZvArMrCeT-_5-oyHjQObc80P04bgUiyRo&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwMFaQ&c=E9plUmAGv24GJC_RgV6lkg&r=ipz9InHENRrvJEfmICylkob51vH0k--_Zwg_B4oxt68&m=reSnEx9x60Sn7qDJL7RV7v4jMeXHB7sTahlobD4ajts&s=WWb8lSKEB7ZvArMrCeT-_5-oyHjQObc80P04bgUiyRo&e=%3e>


Thank you,
Weixi




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