[Histonet] Freezing spray in cryostats
Charles Riley
criley at dpspa.com
Wed Sep 25 13:00:53 CDT 2019
I have to agree with Terri. I also believe that CAP is going to be putting
in regulations forbidding the use of freeze sprays in cryostats soon (at
least I think I heard a rumor about this).
Anyway another useful item is liquid nitrogen if you really need a quick
freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
always recommended as could cause artifacts.
On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> My question is why would you ever want to use an aerosol in the cryostat,
> taking a chance on aerosolizing some nasty bug?
> Is the 30 seconds you save in using freezing spray worth the exposure to
> TB or goodness-knows-what?
> We use a simple metal heat extractor (comes standard in our Leicas) and
> place it on top of the freezing block to accelerate the process. We never
> have issues with turn around times.
> For me, no freezing spray. Ever.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> -----Original Message-----
> From: histonet-request at lists.utsouthwestern.edu [mailto:
> histonet-request at lists.utsouthwestern.edu]
> Sent: Wednesday, September 25, 2019 1:00 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 190, Issue 18
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> Today's Topics:
>
> 1. Flammable Sprays in Cryostats (Knutson, Deanne)
> 2. Re: Flammable Sprays in Cryostats (Akemi)
> 3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
> 4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 25 Sep 2019 07:50:44 -0500
> From: "Knutson, Deanne" <DKnutson at primecare.org>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Flammable Sprays in Cryostats
> Message-ID:
> <
> 1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I just received a letter from Leica Biosystems where they are prohibiting
> the usage of flammable
> freezing sprays in their cryostats. What are others using in their
> cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknutson at primecare.org<mailto:dknutson at primecare.org>
>
>
>
>
>
>
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> ------------------------------
>
> Message: 2
> Date: Wed, 25 Sep 2019 09:05:20 -0500
> From: Akemi <akemiat3377 at gmail.com>
> To: "Knutson, Deanne" <DKnutson at primecare.org>, Histonet
> <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Flammable Sprays in Cryostats
> Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com>
> Content-Type: text/plain; charset=us-ascii
>
> We are purchasing two of their newer UV cryostats and they were adamant
> that we use a non flammable freezing spray because it can damage the UV
> unit and have potential for exploding.
>
> Akemi Allison
>
> Sent from my iPhone
>
> > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable
> > freezing sprays in their cryostats. What are others using in their
> cryostats to instantly freeze specimens?
> >
> > Thank you.
> >
> >
> > Deanne Knutson
> > Supervisor
> > Anatomic Pathology
> > dknutson at primecare.org<mailto:dknutson at primecare.org>
> >
> >
> >
> >
> >
> >
> > ________________________________
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>
> ------------------------------
>
> Message: 3
> Date: Wed, 25 Sep 2019 15:49:00 +0000
> From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
> To: "'Knutson, Deanne'" <DKnutson at primecare.org>
> Cc: "Histonet at lists.utsouthwestern.edu"
> <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Flammable Sprays in Cryostats
> Message-ID:
> <68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We received the same letter months ago. We changed to a non-flammable
> spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog
> number is SL202/134a. Be careful if you order it because they also sell a
> product called STATFREEZE that is flammable.
>
>
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.hannen at parrishmed.com
> www.parrishmed.com
>
>
>
> -----Original Message-----
> From: Knutson, Deanne via Histonet [mailto:
> histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, September 25, 2019 8:51 AM
> To: 'histonet at lists.utsouthwestern.edu'
> Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats
>
>
> This message came from an external source. Please do not click links or
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> Begin Original Message:
>
> ----------------------------------------------------------------------
> I just received a letter from Leica Biosystems where they are prohibiting
> the usage of flammable
>
> freezing sprays in their cryostats. What are others using in their
> cryostats to instantly freeze specimens?
>
>
>
> Thank you.
>
>
>
>
>
> Deanne Knutson
>
> Supervisor
>
> Anatomic Pathology
>
> dknutson at primecare.org<mailto:dknutson at primecare.org>
>
>
>
>
>
>
>
>
>
>
>
>
>
> ________________________________
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> This email may include confidential and privileged information. If this is
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> ------------------------------
>
> Message: 4
> Date: Wed, 25 Sep 2019 16:42:30 +0000
> From: Ingles Claire <CIngles at uwhealth.org>
> To: "'histonet at lists.utsouthwestern.edu'"
> <Histonet at lists.utsouthwestern.edu>, "Knutson, Deanne"
> <DKnutson at primecare.org>
> Subject: Re: [Histonet] Flammable Sprays in Cryostats
> Message-ID:
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> Liquid Nitrogen guns.
> Claire
> ________________________________
> From: Knutson, Deanne via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, September 25, 2019 7:50 AM
> To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu
> >
> Subject: [Histonet] Flammable Sprays in Cryostats
>
> WARNING: This email appears to have originated outside of the UW Health
> email system.
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>
>
>
> I just received a letter from Leica Biosystems where they are prohibiting
> the usage of flammable
> freezing sprays in their cryostats. What are others using in their
> cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknutson at primecare.org<mailto:dknutson at primecare.org>
>
>
>
>
>
>
> ________________________________
> This email may include confidential and privileged information. If this is
> not intended for your use, please destroy immediately and contact the
> sender of the message.
>
> This email and attachments contain information that may be confidential or
> privileged. If you are not the intended recipient, notify the sender at
> once and delete this message completely from your information system.
> Further use, disclosure, or copying of information contained in this email
> is not authorized, and any such action should not be construed as a waiver
> of privilege or other confidentiality protections.
> _______________________________________________
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--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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