[Histonet] Freezing spray in cryostats
Terri Braud
tbraud at holyredeemer.com
Wed Sep 25 12:31:15 CDT 2019
My question is why would you ever want to use an aerosol in the cryostat, taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or goodness-knows-what?
We use a simple metal heat extractor (comes standard in our Leicas) and place it on top of the freezing block to accelerate the process. We never have issues with turn around times.
For me, no freezing spray. Ever.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu]
Sent: Wednesday, September 25, 2019 1:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 190, Issue 18
CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments.
Send Histonet mailing list submissions to
histonet at lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request at lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner at lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Flammable Sprays in Cryostats (Knutson, Deanne)
2. Re: Flammable Sprays in Cryostats (Akemi)
3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
4. Re: Flammable Sprays in Cryostats (Ingles Claire)
----------------------------------------------------------------------
Message: 1
Date: Wed, 25 Sep 2019 07:50:44 -0500
From: "Knutson, Deanne" <DKnutson at primecare.org>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Flammable Sprays in Cryostats
Message-ID:
<1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org>
Content-Type: text/plain; charset="us-ascii"
I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable
freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens?
Thank you.
Deanne Knutson
Supervisor
Anatomic Pathology
dknutson at primecare.org<mailto:dknutson at primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
------------------------------
Message: 2
Date: Wed, 25 Sep 2019 09:05:20 -0500
From: Akemi <akemiat3377 at gmail.com>
To: "Knutson, Deanne" <DKnutson at primecare.org>, Histonet
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com>
Content-Type: text/plain; charset=us-ascii
We are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding.
Akemi Allison
Sent from my iPhone
> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable
> freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknutson at primecare.org<mailto:dknutson at primecare.org>
>
>
>
>
>
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Wed, 25 Sep 2019 15:49:00 +0000
From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
To: "'Knutson, Deanne'" <DKnutson at primecare.org>
Cc: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID:
<68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local>
Content-Type: text/plain; charset="us-ascii"
We received the same letter months ago. We changed to a non-flammable spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog number is SL202/134a. Be careful if you order it because they also sell a product called STATFREEZE that is flammable.
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com
-----Original Message-----
From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, September 25, 2019 8:51 AM
To: 'histonet at lists.utsouthwestern.edu'
Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats
This message came from an external source. Please do not click links or open attachments if unexpected or unusual.
Begin Original Message:
----------------------------------------------------------------------
I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable
freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens?
Thank you.
Deanne Knutson
Supervisor
Anatomic Pathology
dknutson at primecare.org<mailto:dknutson at primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=rqHf4UNcHbvp592ujkRAO5WLlEJw9KUFgBy00GsLhqs&s=aDgPVL1SLa_CnToePuo4VME9QjJOweGzyLEoVKJSWPg&e=
------------------------------
Message: 4
Date: Wed, 25 Sep 2019 16:42:30 +0000
From: Ingles Claire <CIngles at uwhealth.org>
To: "'histonet at lists.utsouthwestern.edu'"
<Histonet at lists.utsouthwestern.edu>, "Knutson, Deanne"
<DKnutson at primecare.org>
Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID:
<DM5PR1701MB191313D76EF3A406C8C73F82C9870 at DM5PR1701MB1913.namprd17.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
Liquid Nitrogen guns.
Claire
________________________________
From: Knutson, Deanne via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Flammable Sprays in Cryostats
WARNING: This email appears to have originated outside of the UW Health email system.
DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.
I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable
freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens?
Thank you.
Deanne Knutson
Supervisor
Anatomic Pathology
dknutson at primecare.org<mailto:dknutson at primecare.org>
________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Subject: Digest Footer
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
End of Histonet Digest, Vol 190, Issue 18
*****************************************
More information about the Histonet
mailing list