[Histonet] Freezing spray in cryostats

Akemi akemiat3377 at gmail.com
Wed Sep 25 13:25:35 CDT 2019


I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential TB and regular masks for normal tissue.
Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I have to agree with Terri. I also believe that CAP is going to be putting
> in regulations forbidding the use of freeze sprays in cryostats soon (at
> least I think I heard a rumor about this).
> 
> Anyway another useful item is liquid nitrogen if you really need a quick
> freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
> always recommended as could cause artifacts.
> 
> On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> 
>> My question is why would you ever want to use an aerosol in the cryostat,
>> taking a chance on aerosolizing some nasty bug?
>> Is the 30 seconds you save in using freezing spray worth the exposure to
>> TB or goodness-knows-what?
>> We use a simple metal heat extractor (comes standard in our Leicas) and
>> place it on top of the freezing block to accelerate the process.  We never
>> have issues with turn around times.
>> For me, no freezing spray.  Ever.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -----Original Message-----
>> From: histonet-request at lists.utsouthwestern.edu [mailto:
>> histonet-request at lists.utsouthwestern.edu]
>> Sent: Wednesday, September 25, 2019 1:00 PM
>> To: histonet at lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 190, Issue 18
>> 
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>> Today's Topics:
>> 
>>   1. Flammable Sprays in Cryostats (Knutson, Deanne)
>>   2. Re: Flammable Sprays in Cryostats (Akemi)
>>   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
>>   4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>> 
>> 
>> ----------------------------------------------------------------------
>> 
>> Message: 1
>> Date: Wed, 25 Sep 2019 07:50:44 -0500
>> From: "Knutson, Deanne" <DKnutson at primecare.org>
>> To: "'histonet at lists.utsouthwestern.edu'"
>>        <histonet at lists.utsouthwestern.edu>
>> Subject: [Histonet] Flammable Sprays in Cryostats
>> Message-ID:
>>        <
>> 1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org>
>> 
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> I just received a letter from Leica Biosystems where they are prohibiting
>> the usage of flammable
>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>> 
>> Thank you.
>> 
>> 
>> Deanne Knutson
>> Supervisor
>> Anatomic Pathology
>> dknutson at primecare.org<mailto:dknutson at primecare.org>
>> 
>> 
>> 
>> 
>> 
>> 
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>> 
>> ------------------------------
>> 
>> Message: 2
>> Date: Wed, 25 Sep 2019 09:05:20 -0500
>> From: Akemi <akemiat3377 at gmail.com>
>> To: "Knutson, Deanne" <DKnutson at primecare.org>, Histonet
>>        <Histonet at lists.utsouthwestern.edu>
>> Subject: Re: [Histonet] Flammable Sprays in Cryostats
>> Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com>
>> Content-Type: text/plain;       charset=us-ascii
>> 
>> We are purchasing two of their newer UV cryostats and they were adamant
>> that we use a non flammable freezing spray because it can damage the UV
>> unit and have potential for exploding.
>> 
>> Akemi Allison
>> 
>> Sent from my iPhone
>> 
>>> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
>> histonet at lists.utsouthwestern.edu> wrote:
>>> 
>>> I just received a letter from Leica Biosystems where they are
>> prohibiting the usage of flammable
>>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>>> 
>>> Thank you.
>>> 
>>> 
>>> Deanne Knutson
>>> Supervisor
>>> Anatomic Pathology
>>> dknutson at primecare.org<mailto:dknutson at primecare.org>
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> ________________________________
>>> This email may include confidential and privileged information. If this
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>> 
>> 
>> ------------------------------
>> 
>> Message: 3
>> Date: Wed, 25 Sep 2019 15:49:00 +0000
>> From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
>> To: "'Knutson, Deanne'" <DKnutson at primecare.org>
>> Cc: "Histonet at lists.utsouthwestern.edu"
>>        <Histonet at lists.utsouthwestern.edu>
>> Subject: Re: [Histonet] Flammable Sprays in Cryostats
>> Message-ID:
>>        <68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local>
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> We received the same letter months ago.  We changed to a non-flammable
>> spray.  It is called STATFREEZE, we purchase it from STATLAB.  The catalog
>> number is SL202/134a.  Be careful if you order it because they also sell a
>> product called STATFREEZE that is flammable.
>> 
>> 
>> 
>> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
>> Section Chief, Histology
>> Parrish Medical Center
>> 951 N. Washington Ave.
>> Titusville,Florida 32796
>> T: (321)268-6333 ext. 7506
>> F: (321) 268-6149
>> valerie.hannen at parrishmed.com
>> www.parrishmed.com
>> 
>> 
>> 
>> -----Original Message-----
>> From: Knutson, Deanne via Histonet [mailto:
>> histonet at lists.utsouthwestern.edu]
>> Sent: Wednesday, September 25, 2019 8:51 AM
>> To: 'histonet at lists.utsouthwestern.edu'
>> Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats
>> 
>> 
>> This message came from an external source. Please do not click links or
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>> Begin Original Message:
>> 
>> ----------------------------------------------------------------------
>> I just received a letter from Leica Biosystems where they are prohibiting
>> the usage of flammable
>> 
>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>> 
>> 
>> 
>> Thank you.
>> 
>> 
>> 
>> 
>> 
>> Deanne Knutson
>> 
>> Supervisor
>> 
>> Anatomic Pathology
>> 
>> dknutson at primecare.org<mailto:dknutson at primecare.org>
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> 
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>> 
>> ------------------------------
>> 
>> Message: 4
>> Date: Wed, 25 Sep 2019 16:42:30 +0000
>> From: Ingles Claire <CIngles at uwhealth.org>
>> To: "'histonet at lists.utsouthwestern.edu'"
>>        <Histonet at lists.utsouthwestern.edu>,    "Knutson, Deanne"
>>        <DKnutson at primecare.org>
>> Subject: Re: [Histonet] Flammable Sprays in Cryostats
>> Message-ID:
>>        <
>> DM5PR1701MB191313D76EF3A406C8C73F82C9870 at DM5PR1701MB1913.namprd17.prod.outlook.com
>>> 
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>> Content-Type: text/plain; charset="us-ascii"
>> 
>> Liquid Nitrogen guns.
>> Claire
>> ________________________________
>> From: Knutson, Deanne via Histonet <histonet at lists.utsouthwestern.edu>
>> Sent: Wednesday, September 25, 2019 7:50 AM
>> To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu
>>> 
>> Subject: [Histonet] Flammable Sprays in Cryostats
>> 
>> WARNING: This email appears to have originated outside of the UW Health
>> email system.
>> DO NOT CLICK on links or attachments unless you recognize the sender and
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>> 
>> 
>> 
>> I just received a letter from Leica Biosystems where they are prohibiting
>> the usage of flammable
>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>> 
>> Thank you.
>> 
>> 
>> Deanne Knutson
>> Supervisor
>> Anatomic Pathology
>> dknutson at primecare.org<mailto:dknutson at primecare.org>
>> 
>> 
>> 
>> 
>> 
>> 
>>  ________________________________
>> This email may include confidential and privileged information. If this is
>> not intended for your use, please destroy immediately and contact the
>> sender of the message.
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>> This email and attachments contain information that may be confidential or
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>> once and delete this message completely from your information system.
>> Further use, disclosure, or copying of information contained in this email
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>> of privilege or other confidentiality protections.
>> _______________________________________________
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> 
> 
> -- 
> 
> Charles Riley BS  HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
> _______________________________________________
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