[Histonet] Histonet Digest, Vol 191, Issue 11

Fri Oct 18 18:16:41 CDT 2019

RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure.

Hi there Mark.

Really appreciate your feedback. I  do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble formation, so i have learnt something. However, i use it at a 0.5 % solution. Maybe thats too much as the solution looks a bit soapy, and could also cause lifting of sections im presuming.
Thanks again, keep well.

Best regards
Subash

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Today's Topics:

1. RELIA HOT JOB ALERT! IHC Specialist for New England area.
Contact me for info ASAP! (Pam Barker)
2. How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Donovan, Mark)

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Message: 1
Date: Thu, 17 Oct 2019 14:23:52 -0400
From: "Pam Barker" <relia1 at earthlink.net>
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Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New
England area. Contact me for info ASAP!
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Message: 2
Date: Thu, 17 Oct 2019 23:10:56 +0000
From: "Donovan, Mark" <M.Donovan at alfred.org.au>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
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Sabash,

What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect.

Regards,
Mark

Original message:

Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz <mcruz84pr at gmail.com<mailto:mcruz84pr at gmail.com>>
To: "histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>"
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Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
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Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute.

Maria

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Hi there all

Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks.

Best Regards
Subash Govender

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