[Histonet] Histonet Digest, Vol 191, Issue 12

Sun Oct 20 11:30:46 CDT 2019

Re: How to prevent bubbling in pressure cooker for the immunohistichemistry procedure

Hi there Carl

Thank you so much for your advice. I do actually use Marienfeld Histobond  slides, they are silane coated. So dont think that is the prolem, but i do like your advice of drying the tissue under the fan. The tissues that give me problems are breast, cervix, and other odd tissues. With breast tissue being fatty i suppose you would have a bit of lifting. Another kind lady also gave me the idea of actually placing the slides into the pressure cooker and sealing the lid immediately after being dewaxed instead of waiting for the solution to boil first like i do.
Thanks again for your feedback Carl, much appreciated.
Subash

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Today's Topics:

1. Re: How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Hobbs, Carl)
2. Re: Histonet Digest, Vol 191, Issue 10 (Subash Govender)
3. Re: Histonet Digest, Vol 191, Issue 11 (Subash Govender)

----------------------------------------------------------------------

Message: 1
Date: Fri, 18 Oct 2019 17:58:12 +0000
From: "Hobbs, Carl" <carl.hobbs at kcl.ac.uk>
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
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Hi Subash
Imho your sections are not sufficiently adherent to the slide(s)
It is not M/W "bubbling" that causes your problem
If you use Superfrost Plus/lab-coated Silane or even double subbed slides ( thanks Ole!) AND dry your sections onto the slides efficiently, you will not get section-loss.
NB: SF+ are expensive: if you cannot afford them....experiment with in-house Silane ( APES) or subbing, to coat your IHC slides.
If you use the latter methods, make sure you slides are very clean.
Unless the tissue is very fibrous/bone/cartilage/severely inflamed....difficult to keep sections adherent.
In the latter case, Trajan 3 series can help much more than std coated slides.
What tissues are causing you problems?
For most soft tissues I use Superfrost Plus slides, dry the mounted sections under a fan O/N ( at least one hour)
For my peace of mind I then put the slides into a 60C oven for 30-60 mins before dewaxing.
Please post your opinion/results so far?
Best wishes
Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813

------------------------------

Message: 2
Date: Fri, 18 Oct 2019 23:00:16 +0000
From: Subash Govender <subash.govender at uct.ac.za>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 10
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Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure.

Maria
Thank you so much for your feedback. The instrument i use for the antigen retrieval process is just household 6L Russek Hobbs pressure cooker. Before i place my slides into this PC i do actually wait for it to boil and actually do see the solution bubble. Then only do i put my slides in and close the lid. I am assuming it continues to bubble while reaching its optimum temperature, but you think not, so i am unsure. This procedure works quite well as we get good results. However, i do get an odd 1 or 2 tissues washing off now and again. So to try and stop this completely and to perfect our method i thought about this article i read, but sadly have lost. As i do think that maybe no bubbling will prevent this. But could be wrong. Will keep searching for the info i lost.
Thanks again, keep well.

Best Regards
Subash

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Today's Topics:

1. Re: How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Maria Cruz)
2. Re: ADH5 multiplex procedure (Sally Price)
3. Re: cleaning old charged slides (Atoska Gentry)
4. procuring fresh tumor tissue (Curt Tague)
5. Re: cleaning old charged slides (Dave Juliano)
6. ICYMI here is a link to part 3 of 3 in my series on
Relocation - Relocation an Action Plan. And our latest histology
opportunties on and off the bench! (Pam Barker)

----------------------------------------------------------------------

Message: 1
Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz <mcruz84pr at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:
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Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and
reliable pre-treatment of sections while PREVENTING boiling and potential
tissue detachment, you really shouldn?t be experiencing any bubbling. If
you are, then your PC isn?t sealed adequately and the only way to fix
that problem is to replace the gasket or find another way to tighten the
lid.. I?ve never heard of anything that you can put inside of a PC that
can change the way it pressurizes, so I?ll be following this thread to see
if anyone else can contribute.

Maria

**************************************

Hi there all

Can anybody please tell me if they have heard of or perhaps know of a
substance that can be placed in the pressure cooker during the antigen
retrieval process for immunohistochemistry. This prevents excessive
bubbling and lifting up of sections. I did somehow come across such info a
while back, but seem to have lost it. Many thanks.

Best Regards
Subash Govender

------------------------------

Message: 2
Date: Wed, 16 Oct 2019 15:06:02 -0400
From: Sally Price <sprice2003 at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] ADH5 multiplex procedure
Message-ID:
<CAHonC4Dwo7TKxhngxaVJFSR60Og9y-OQEe6WHDYU8ho9YhM4iQ at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

---------- Forwarded message ----------
From: "Kurth Virginia L." <VKurth at uwhealth.org>
To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu>

Virginia:
I?m pretty certain that the ADH5 multiplex procedure is performed at CSI
Labs in the Atlanta area. And I don?t think that biocare refers to it by
that name any more either - I think they just spell out all the antigens
that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18.

Sally

- - - - - - - - - - -

Date: Tue, 15 Oct 2019 14:48:40

Subject: [Histonet] ADH5
Does anyone know if there is a reference lab that performs ADH5.
(particular the BioCare multiplex stain?)

Thanks Ginny--
Sally Price

------------------------------

Message: 3
Date: Thu, 17 Oct 2019 15:16:58 +0000
From: Atoska Gentry <gentras at auburn.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] cleaning old charged slides
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Hello,

Has anyone had success cleaning old charged slides which have become foggy over time.

Thanks!
~Atoska

------------------------------

Message: 4
Date: Thu, 17 Oct 2019 16:00:18 +0000
From: Curt Tague <c.tague at pathologyarts.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] procuring fresh tumor tissue
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Hello again histonet-ers-

I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology.
Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics?

All the best to you all, thanks for your thoughts,

Curt

------------------------------

Message: 5
Date: Thu, 17 Oct 2019 09:21:09 -0700
From: Dave Juliano <dave.c.juliano at gmail.com>
To: Atoska Gentry <gentras at auburn.edu>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] cleaning old charged slides
Message-ID:
<CAKxzVt+yD0w1Bs2QqDHBc1Jrr5d3TudY=sb9+WN2raLw5N3TPw at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones-
studying effects of age on charges slides. Contact me at: Dave.c.juliano
at gmail dot com if you?re interested!

On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
>
> Has anyone had success cleaning old charged slides which have become foggy
> over time.
>
> Thanks!
> ~Atoska
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
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------------------------------

Message: 6
Date: Thu, 17 Oct 2019 12:27:50 -0400
From: "Pam Barker" <relia1 at earthlink.net>
To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series
on Relocation - Relocation an Action Plan. And our latest histology
opportunties on and off the bench!
Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters,
ICYMI - In case you missed it!!
I am so excited!! My latest article has been published in the NSH Quarterly
Career Center Newsletter under my byline:
Make the Cut
My latest article is entitled: Relocation ? An Action Plan
This is part 3 of 3 in my series on relocation. You can find a link to
parts 1&2 in this article as well!
This is the link to my article on the NSH?s Fixation on Histology Blog and
it is also in the current edition of the NSH Career Newsletter.

Here is the link to the article:
https://www.fixationonhistology.com/post/relocation-part-3-action-plan<https://protect-za.mimecast.com/s/owp1C3lr5wiLPGOoU881lt><https://protect-za.mimecast.com/s/Y4K7CBgX56fEKL03tvyFb_<https://protect-za.mimecast.com/s/owp1C3lr5wiLPGOoU881lt>>

If you have a minute to read it I would love to hear what you think.
If you can?t get to the article with this link let me know and I will send
you a copy of it.
Histopeeps, we also have amazing opportunities nationwide!
Leadership and Specialty opportunities:
1. Chicago Greater Chicago area-Field Applications
Specialist ? IHC
2. Boston Greater Boston area-Field Applications
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6. Orlando Histology Instructor

HT or HTL or elig opportunities:
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2. California Northern CA Histology tech
3. N. Carolina Raleigh/Durham area Histology Tech
4. Colorado Denver area ? IHC Histology Tech
5. Wisconsin Milwaukee ? Histology Tech
6. Georgia Columbus ? Histotech dermpath
7. S. Carolina Beaufort ? Histology Tech

Please take a look and if you are interested let me know. If you have a
friend who is interested and I place them then I get to give you a referral
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My clients offer competitive pay rates, excellent benefits and in most cases
Relocation assistance or a sign on bonus.
They are ready to interview and hire!!!

If You or Anyone You Know Might Be Interested In a New Opportunity, Please
Contact Me ASAP
call or text me on my cell At 407-353-5070.
If you want some additional information or to set up a time to chat please
call me toll free at 866-607-3542 or email me at relia1 at earthlink.net

Have a great day. I look forward to hearing back from you.

Thanks-Pam

#jobs4myhistopeeps
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
?Pam M. Barker?
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788
E-mail: relia1 at earthlink.net
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Message: 3
Date: Fri, 18 Oct 2019 23:16:41 +0000
From: Subash Govender <subash.govender at uct.ac.za>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 11
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RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure.

Hi there Mark.

Really appreciate your feedback. I do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble formation, so i have learnt something. However, i use it at a 0.5 % solution. Maybe thats too much as the solution looks a bit soapy, and could also cause lifting of sections im presuming.
Thanks again, keep well.

Best regards
Subash

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Subject: Histonet Digest, Vol 191, Issue 11

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Today's Topics:

1. RELIA HOT JOB ALERT! IHC Specialist for New England area.
Contact me for info ASAP! (Pam Barker)
2. How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Donovan, Mark)

----------------------------------------------------------------------

Message: 1
Date: Thu, 17 Oct 2019 14:23:52 -0400
From: "Pam Barker" <relia1 at earthlink.net>
To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New
England area. Contact me for info ASAP!
Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters!
I hope you are having a great day and cruising into the weekend. I have an
opportunity that I want to share in hopes that you or someone you know might
be interested.
I am looking for a Field Applications Support Specialist ?IHC for a
territory in New England. This is a RELIA EXCLUSIVE!
My client offers an excellent product line and reputation to support it.
This is a full time salaried position NO sales required.
Use your IHC expertise to help my client?s customers further their support
of patient care.
My client is offering a very competitive salary, travel expenses, benefits
and more.
If you would like details on the opportunity please contact me ASAP!

Thanks-Pam

#jobs4myhistopeeps
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
?Pam M. Barker?
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788
E-mail: relia1 at earthlink.net
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
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------------------------------

Message: 2
Date: Thu, 17 Oct 2019 23:10:56 +0000
From: "Donovan, Mark" <M.Donovan at alfred.org.au>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
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Sabash,

What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect.

Regards,
Mark

Original message:

Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz <mcruz84pr at gmail.com<mailto:mcruz84pr at gmail.com>>
To: "histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>"
<histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:
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Content-Type: text/plain; charset="UTF-8"

Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute.

Maria

**************************************

Hi there all

Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks.

Best Regards
Subash Govender

------------------------------

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End of Histonet Digest, Vol 191, Issue 12
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