[Histonet] Histonet Digest, Vol 191, Issue 10

Fri Oct 18 18:00:16 CDT 2019

Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure.

Maria
Thank you so much for your feedback. The instrument i use for the antigen retrieval process is just household 6L Russek Hobbs pressure cooker. Before i place my slides into this PC i do actually wait for it to boil and actually do see the solution bubble. Then only do i put my slides in and close the lid. I am assuming it continues to bubble while reaching its optimum temperature, but you think not, so i am unsure. This procedure works quite well as we get good results. However, i do get an odd 1 or 2 tissues washing off now and again. So to try and stop this completely and to perfect our method i thought about this article i read, but sadly have lost. As i do think that maybe no bubbling will prevent this. But could be wrong. Will keep searching for the info i lost.
Thanks again, keep well.

Best Regards
Subash

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Sent: Thursday, October 17, 2019 7:00:01 PM
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Subject: Histonet Digest, Vol 191, Issue 10

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Today's Topics:

1. Re: How to prevent bubbling in pressure cooker for
immunohistochemistry procedure (Maria Cruz)
2. Re: ADH5 multiplex procedure (Sally Price)
3. Re: cleaning old charged slides (Atoska Gentry)
4. procuring fresh tumor tissue (Curt Tague)
5. Re: cleaning old charged slides (Dave Juliano)
6. ICYMI here is a link to part 3 of 3 in my series on
Relocation - Relocation an Action Plan. And our latest histology
opportunties on and off the bench! (Pam Barker)

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Message: 1
Date: Wed, 16 Oct 2019 13:43:28 -0400
From: Maria Cruz <mcruz84pr at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for
immunohistochemistry procedure
Message-ID:
<CAPg7ww1f0dsBw85M8wNRZ2PAMxpgKqPxw0V9Kx-SVxMWbV1m1A at mail.gmail.com>
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Subash:
Since the main purpose of using a pressure cooker for HIER is the rapid and
reliable pre-treatment of sections while PREVENTING boiling and potential
tissue detachment, you really shouldn?t be experiencing any bubbling. If
you are, then your PC isn?t sealed adequately and the only way to fix
that problem is to replace the gasket or find another way to tighten the
lid.. I?ve never heard of anything that you can put inside of a PC that
can change the way it pressurizes, so I?ll be following this thread to see
if anyone else can contribute.

Maria

**************************************

Hi there all

Can anybody please tell me if they have heard of or perhaps know of a
substance that can be placed in the pressure cooker during the antigen
retrieval process for immunohistochemistry. This prevents excessive
bubbling and lifting up of sections. I did somehow come across such info a
while back, but seem to have lost it. Many thanks.

Best Regards
Subash Govender

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Message: 2
Date: Wed, 16 Oct 2019 15:06:02 -0400
From: Sally Price <sprice2003 at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] ADH5 multiplex procedure
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---------- Forwarded message ----------
From: "Kurth Virginia L." <VKurth at uwhealth.org>
To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu>

Virginia:
I?m pretty certain that the ADH5 multiplex procedure is performed at CSI
Labs in the Atlanta area. And I don?t think that biocare refers to it by
that name any more either - I think they just spell out all the antigens
that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18.

Sally

- - - - - - - - - - -

Date: Tue, 15 Oct 2019 14:48:40

Subject: [Histonet] ADH5
Does anyone know if there is a reference lab that performs ADH5.
(particular the BioCare multiplex stain?)

Thanks Ginny--
Sally Price

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Message: 3
Date: Thu, 17 Oct 2019 15:16:58 +0000
From: Atoska Gentry <gentras at auburn.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] cleaning old charged slides
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Hello,

Has anyone had success cleaning old charged slides which have become foggy over time.

Thanks!
~Atoska

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Message: 4
Date: Thu, 17 Oct 2019 16:00:18 +0000
From: Curt Tague <c.tague at pathologyarts.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] procuring fresh tumor tissue
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Hello again histonet-ers-

I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology.
Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics?

All the best to you all, thanks for your thoughts,

Curt

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Message: 5
Date: Thu, 17 Oct 2019 09:21:09 -0700
From: Dave Juliano <dave.c.juliano at gmail.com>
To: Atoska Gentry <gentras at auburn.edu>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] cleaning old charged slides
Message-ID:
<CAKxzVt+yD0w1Bs2QqDHBc1Jrr5d3TudY=sb9+WN2raLw5N3TPw at mail.gmail.com>
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Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones-
studying effects of age on charges slides. Contact me at: Dave.c.juliano
at gmail dot com if you?re interested!

On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
>
> Has anyone had success cleaning old charged slides which have become foggy
> over time.
>
> Thanks!
> ~Atoska
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/i7J9CAnX51i7ODBMtMu1Qc>
>

------------------------------

Message: 6
Date: Thu, 17 Oct 2019 12:27:50 -0400
From: "Pam Barker" <relia1 at earthlink.net>
To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series
on Relocation - Relocation an Action Plan. And our latest histology
opportunties on and off the bench!
Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonetters,
ICYMI - In case you missed it!!
I am so excited!! My latest article has been published in the NSH Quarterly
Career Center Newsletter under my byline:
Make the Cut
My latest article is entitled: Relocation ? An Action Plan
This is part 3 of 3 in my series on relocation. You can find a link to
parts 1&2 in this article as well!
This is the link to my article on the NSH?s Fixation on Histology Blog and
it is also in the current edition of the NSH Career Newsletter.

Here is the link to the article:
https://www.fixationonhistology.com/post/relocation-part-3-action-plan<https://protect-za.mimecast.com/s/Y4K7CBgX56fEKL03tvyFb_>

If you have a minute to read it I would love to hear what you think.
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Histopeeps, we also have amazing opportunities nationwide!
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If You or Anyone You Know Might Be Interested In a New Opportunity, Please
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Have a great day. I look forward to hearing back from you.

Thanks-Pam

#jobs4myhistopeeps
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Follow my hashtags and make your day great and your career greater!!

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?Pam M. Barker?
Pam Barker
President/Senior Recruiting Specialist-Histology
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Phone: (407)657-2027
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E-mail: relia1 at earthlink.net
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End of Histonet Digest, Vol 191, Issue 10
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