[Histonet] Procedure for Blue Cheese for Fungus Controls

[John Garratt]

If you have access to spare fresh tissue (ie placenta) you can infuse the tissue with fungus (blue cheese if you wish) and cultivate overnight before FFPE, I have found these make excellent control blocks and the tissue makes for good structural support, plus the tissue architecture might make the blue cheese more palatable to pathologists. Your idea is certainly not crackers.

John


www.ciqc.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Friday, November 22, 2019 5:01 PM, Akemi via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hi Histopeeps! Here’s my procedure for Fungus Test Controls since we are out of controls. This was from Blue Cheese I processed yesterday. It turned out fabulous!!! Photo credit to Christina George (i know Histonet doesn’t attach photos so can send to you) and lab credit to my staff, Quinnlan Dewitt and Carlos Duran. I used President brand Le Blue which I purchased from Sprouts grocery. It cost $7.95 for 3.5 oz. it was the most aged. I then took a portion of the “moldy blue” cheese and put it in lens paper, then cassetted it and fixed it in 10% Formalin in the morning for 8 hours and ran it normally with routine surgicals. Thinking of publishing!
>
> Cheers!
>
> Sent from my iPhone
>
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