[Histonet] Use of Freeze Spray in a Cryostat

Erin McCarthy erin.mccarthy at tempus.com
Tue May 28 12:19:32 CDT 2019

Hi Jennifer -

It was my understanding that the recall was particularly related to the
flammability of the freeze spray. If you can find a spray with out a
flammability hazard you can keep using the spray. I don't have a lot of
frozen sections in my current job. But in my last job, and my experience in
hospitals to get rapid results for patients still on the table sprays are
used. In cases of TB they are discouraged.

On Tue, May 28, 2019 at 12:12 PM Schumacher, Jennifer J via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi all,
> Looking for some documentation regarding the use of Freeze Spray in
> cryostats.
> A Best Practice document would be ideal, but in the absence of such a
> document, I would like to hear how others are planning to address the
> "recall" by Leica where they do not recommend the use of the Freeze Spray
> in their cryostats due to the possibility of a fire (flammable reagent).
> I personally have always had concerns around infection since the specimens
> are fresh and using the spray could aerosolize any infectious agents that
> are present.
> There is a lot of pressure to freeze things rapidly to meet the FS TAT, so
> the battle goes back and forth.
> Any feedback is much appreciated.
> Jennifer
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Erin McCarthy, HT (ASCP)
Histology Supervisor

Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
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