[Histonet] Use of Freeze Spray in a Cryostat

Victoria Baker bakevictoria at gmail.com
Tue May 28 13:43:38 CDT 2019


I called Leica as we have 2 cryostats at different locations so we needed
to be sure that we could use our current spray that we get from Leica.

We were told that their spray with the 1,1,1,2 Tetrafluoroethane is safe to
I would therefore check to see what you have in stock and if it is this you
should follow the guidelines on the can.

We do not use spray on possible airborne pathogen cases and we avoid
spraying the specimen directly at all times.

Hope this helps.


On Tue, May 28, 2019, 1:11 PM Schumacher, Jennifer J via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi all,
> Looking for some documentation regarding the use of Freeze Spray in
> cryostats.
> A Best Practice document would be ideal, but in the absence of such a
> document, I would like to hear how others are planning to address the
> "recall" by Leica where they do not recommend the use of the Freeze Spray
> in their cryostats due to the possibility of a fire (flammable reagent).
> I personally have always had concerns around infection since the specimens
> are fresh and using the spray could aerosolize any infectious agents that
> are present.
> There is a lot of pressure to freeze things rapidly to meet the FS TAT, so
> the battle goes back and forth.
> Any feedback is much appreciated.
> Jennifer
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