[Histonet] Use of Freeze Spray in a Cryostat

[Schumacher, Jennifer J]

Hi all,
Looking for some documentation regarding the use of Freeze Spray in cryostats.

A Best Practice document would be ideal, but in the absence of such a document, I would like to hear how others are planning to address the "recall" by Leica where they do not recommend the use of the Freeze Spray in their cryostats due to the possibility of a fire (flammable reagent).

I personally have always had concerns around infection since the specimens are fresh and using the spray could aerosolize any infectious agents that are present.

There is a lot of pressure to freeze things rapidly to meet the FS TAT, so the battle goes back and forth.

Any feedback is much appreciated.

Jennifer


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