[Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline

[Jan Shivers]

Hi Sandra,

Being a mouse monoclonal, it may not cross-react with canines/felines; the
epitope may be specific to human follicular cells only.

The polyclonal Thyroglobulin from Dako/Agilent works fine on all the
mammals I've tried it on so far (and a frog as well).

Regards,
Jan

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 at umn.edu

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On Wed, May 15, 2019 at 10:54 AM Sandra Cheasty via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
>                 We had to switch sources (to Novus) for Thyroglobulin
> TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet
> suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and
> pH9 EDTA AR, to no avail. Any suggestions?
> Thanks!
> Sandy
>
> Sandra J. Cheasty, HT (ASCP)
> Histology & Necropsy Supervisor
> UW-Madison, School of Veterinary Medicine
>
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