[Histonet] K and L

[Kuhnla, Melissa]

Good Afternoon,
We are recently experiencing some staining issues with our IHC kappa and lambda.  These antibodies have historically been problematic.  We are currently running cell marque clones EP171 and EP 172 with Roche ultraview detection.  The issues are complicated as sometimes they contradict each other.  Lambda has too much background, which should be easy enough to remedy.  Both stains also just recently have a lack of target specificity.  Only recently we have had multiple cases either both k and l are negative or both stains are positive.  Anyone experiencing anything similar.  Thank you in advance.

Melissa Kuhnla
Lead Medical Technologist
for IHC and FISH testing
Regional Laboratory Services
Good Samaritan Hospital
70 Arkay Dr.
Hauppauge, NY 11788
631-609-2551

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