[Histonet] K and L

[Eddie Martin]

Hi Melissa, 

It is possible to have a double negative K/L depending on patient’s diagnosis. It is also normal to have positive K/L expression in tissue samples. 

They are historically dirty in IHC. But you would have more control if you optimized with a concentrate version of the antibody instead of a Ready To Use. If you decide to still use the ready to use, you will have to add a lot of additional steps to reduce the background stain. 

Eddie Martin
Hematology & Histology Team Lead
The National Institutes Of Health
10 Center Dr
Bethesda, Maryland 20892


> On Jun 18, 2019, at 1:19 PM, Kuhnla, Melissa <Melissa.Kuhnla at chsli.org> wrote:
> 
> Good Afternoon,
> We are recently experiencing some staining issues with our IHC kappa and lambda.  These antibodies have historically been problematic.  We are currently running cell marque clones EP171 and EP 172 with Roche ultraview detection.  The issues are complicated as sometimes they contradict each other.  Lambda has too much background, which should be easy enough to remedy.  Both stains also just recently have a lack of target specificity.  Only recently we have had multiple cases either both k and l are negative or both stains are positive.  Anyone experiencing anything similar.  Thank you in advance.
> 
> Melissa Kuhnla
> Lead Medical Technologist
> for IHC and FISH testing
> Regional Laboratory Services
> Good Samaritan Hospital
> 70 Arkay Dr.
> Hauppauge, NY 11788
> 631-609-2551
> 
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