[Histonet] Sectioning over processed GI biopsy

[Cooper, Brian]

Did you try soaking them face-down on your flotation bath for 20 seconds or so, prior to placing on a very wet ice bath (over a Labsorb or paper towel)?  It sounds like these GI's were processed with an inappropriate protocol on your tissue processor.

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcooper at chla.usc.edu 

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, June 18, 2019 9:49 AM
To: Histo List
Subject: [Histonet] Sectioning over processed GI biopsy (EXTERNAL EMAIL)

What is the best way to get a section from an over-processed GI biopsy?
Paraffin keeps pulling away from the tissue and nothing sections. Tissue
very hard even after lenghty time on ice water bath

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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