[Histonet] IHC mouse optomization

[Margaryan, Naira]

Biocare Medical has pure ready to use secondary.. 

Naira 

-----Original Message-----
From: Blanca Lopez <Blanca.Lopez at UTSouthwestern.edu> 
Sent: Wednesday, June 12, 2019 10:57 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC mouse optomization

Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit HRP has a mixture of mouse and rabbit so we think that might be the reason become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you can share your best procedures in IHC mouse stains. I can have a different ways and products to try. Any suggestion or opinions count. If you have any website that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>



________________________________

UT Southwestern


Medical Center



The future of medicine, today.