[Histonet] IHC mouse optomization

[Colleen Forster]

Biocare Medical Promark series are also great animal polymer based
detection that work very well.

https://biocare.net/products/detection/

Colleen Forster HT(ASCP)WQIUC
U of MN

On Wed, Jun 12, 2019 at 10:42 AM Paula Keene Pierce via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Vector Labs ImmPress kits rock for animal IHC.
> https://vectorlabs.com/browse/immpress-polymer-detection-kits-for-ihc
>
>
>
>
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830
> N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com
>
>     On Wednesday, June 12, 2019, 10:13:48 AM CDT, Blanca Lopez via
> Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
>  Morning,
> I need a good advice in optimizing Mouse antibodies for research purposes.
> I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times.
> Dako kit HRP has a mixture of mouse and rabbit so we think that might be
> the reason become negative.
> Is there any tips for optimizing antibodies special for mouse tissue? Or
> if you can share your best procedures in IHC mouse stains. I can have a
> different ways and products to try. Any suggestion or opinions count. If
> you have any website that I can learn more about IHC for research mainly
> done in  Xenograft.
> Thank you everybody:)
>
> Blanca Lopez HT (ASCP)cm
> Senior Histotechnologist
> UT Southwestern Medical Center
> Harold C. Simmons Comprehensive Cancer Center
> UTSTR Biorepository Tissue Lab
> 6000 Harry Hines Blvd NB5.102
> Dallas, Texas 75390
> 214-648-7598
> blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>
>
>
>
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB      612-626-1930

*If submitting histology request please also forward to Lori Holm at
holml at umn.edu <holml at umn.edu>*