[Histonet] IHC mouse optomization

[Ana Maluenda]

Hi Blanca,

It all depends on what you are trying to stain, how the tissue has been harvested/processed and what are your antibodies. If you are trying to use a primary mouse on mouse tissue, that is always tricky. You will need to probably explore your options with M.O.M kits. Otherwise, you may need to check your primary and secondaries. If you are using a secondary that is rat anti-mouse, you may also need to use an absorbed mouse secondary, to avoid too much non-specific staining.

Kind regards,

Ana

Ana Maluenda
Research Assistant/Laboratory Manager
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au


-----Original Message-----
From: Margaryan, Naira <naira.margaryan at hsc.wvu.edu>
Sent: Friday, 14 June 2019 3:27 AM
To: Blanca Lopez <Blanca.Lopez at UTSouthwestern.edu>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC mouse optomization

Biocare Medical has pure ready to use secondary..

Naira

-----Original Message-----
From: Blanca Lopez <Blanca.Lopez at UTSouthwestern.edu>
Sent: Wednesday, June 12, 2019 10:57 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC mouse optomization

Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit HRP has a mixture of mouse and rabbit so we think that might be the reason become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you can share your best procedures in IHC mouse stains. I can have a different ways and products to try. Any suggestion or opinions count. If you have any website that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>



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