[Histonet] Victoria blue for lung tissue

[Tony Henwood (SCHN)]

Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue:

Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148–149

Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78

Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12

Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Bob Richmond via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Saturday, July 27, 2019 4:10 AM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
lung tissue to exam thickness of artery. Could anybody recommend a good
vendor of this reagent kit?<<

You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
several others. I couldn't find anyone who offers a kit, only the dry dye.
There are other dyes called Victoria Blue, reported to give the same
results.

You'd have to find a method for preparing it as an elastic stain, and I
couldn't find such a method either with Google or in my old books. The
requester needs to supply you with a method. I suspect the requester is
reading an old article. There are stains for elastic tissue (which is I
suppose what you want to "exam thickness of artery") that are a lot easier
to get.

Bob Richmond
Samurai Pathologist
Maryville TN
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