[Histonet] Victoria blue for lung tissue

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)]

Miller's stain is available from VWR.
I do not recommend the BSS to stain elastin - it is not strong enough and misses the fine fibres.

Kind regards,
Penny :)

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.marr at nhs.net

(01424) 758023


-----Original Message-----
From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au]
Sent: 27 July 2019 01:44
To: Bob Richmond
Cc: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue:

Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149

Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78

Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12

Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Bob Richmond via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Saturday, July 27, 2019 4:10 AM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Victoria blue for lung tissue

Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
lung tissue to exam thickness of artery. Could anybody recommend a good
vendor of this reagent kit?<<

You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
several others. I couldn't find anyone who offers a kit, only the dry dye.
There are other dyes called Victoria Blue, reported to give the same
results.

You'd have to find a method for preparing it as an elastic stain, and I
couldn't find such a method either with Google or in my old books. The
requester needs to supply you with a method. I suspect the requester is
reading an old article. There are stains for elastic tissue (which is I
suppose what you want to "exam thickness of artery") that are a lot easier
to get.

Bob Richmond
Samurai Pathologist
Maryville TN
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