[Histonet] Tissue Adherence Issue

[Dorothy Hu]

>
>  2. Tissue Adherence Issue (Knutson, Deanne)
>

Hello,

I think your testing tissue might be over or under fixed in formaldehyde
fixative?
You didn't tell what kind of tissue though. We deal with bone tissue which
needs extensively bake (at 37, 46 degree over night and 58 one hour) and
carefully handled. Superfrost Plus slides are used. Especially gentle heat
for heat induced antigen retrieval. Try to use enzyme antigen retrieval as
possible.
Best luck.

Dorothy Hu



> Message: 2
> Date: Wed, 17 Jul 2019 09:21:49 -0500
> From: "Knutson, Deanne" <DKnutson at primecare.org>
> To: "'histonet at lists.utsouthwestern.edu'"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Tissue Adherence Issue
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>
> Fellow Histonetters -
>
> I would appreciate your feedback on an intermittent issue that has shown
> up in our lab.
> All of a sudden, we are having difficulty with tissue specimens falling
> off of our slides on the IHC stains and special stains sporadically.
> About a year ago, we switched instruments on the IHC bench from the Leica
> BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
> No adherence issues with all of the validation slides that were run.
> We have tried various types of slides recently as well, and the issue
> still prevails.
>
> Would any of you mind telling me your slide workflow - type of slide used,
> gelatin or not used in flotation bath, how long slides are cooked, etc....
> for your IHC slides, for your special stains slides, and even for your H&E
> slides.
> I would welcome your suggestions and feedback.
>
> Thank you so much!!!
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
>
>  [X]
>  "Let All Be Received as Christ."
>
>
>