[Histonet] Tissue Adherence Issue

[Knutson, Deanne]

Thank you to everyone who responded back to my posting.  There were so many great suggestions and ideas for us to investigate.
Who better to reach out to for help on issues than our peers?! - You are awesome!

THANK YOU!!!!!

Deanne Knutson
Supervisor
Anatomic Pathology
 dknutson at primecare.org

"Let All Be Received as Christ."

-----Original Message-----
From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, July 17, 2019 9:22 AM
To: 'histonet at lists.utsouthwestern.edu'
Subject: [Histonet] Tissue Adherence Issue


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Fellow Histonetters -



I would appreciate your feedback on an intermittent issue that has shown up in our lab.

All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically.

About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.

No adherence issues with all of the validation slides that were run.

We have tried various types of slides recently as well, and the issue still prevails.



Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides.

I would welcome your suggestions and feedback.



Thank you so much!!!



Deanne Knutson

Supervisor

Anatomic Pathology



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