[Histonet] Tissue Adherence Issue

[Dilts,Andrew]

We find that the amount of time slides spend baking in the oven prior to deparaffinization on our Benchmark Ultras tends to make a difference.  Harder tissue like brain and bone can even benefit from  2 hours at 58*C.



Andrew Dilts  HTL(ASCP)
Histo Supervisor, Laboratory Services
Phone: (417) 269-5021
Andrew.Dilts at coxhealth.com
www.coxhealth.com



-----Original Message-----
From: Knutson, Deanne <DKnutson at primecare.org>
Sent: Wednesday, July 17, 2019 9:22 AM
To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Tissue Adherence Issue

Fellow Histonetters -

I would appreciate your feedback on an intermittent issue that has shown up in our lab.
All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically.
About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
No adherence issues with all of the validation slides that were run.
We have tried various types of slides recently as well, and the issue still prevails.

Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides.
I would welcome your suggestions and feedback.

Thank you so much!!!

Deanne Knutson
Supervisor
Anatomic Pathology

 [X]
 "Let All Be Received as Christ."



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