[Histonet] Tissue Adherence Issue

[Knutson, Deanne]

Fellow Histonetters -

I would appreciate your feedback on an intermittent issue that has shown up in our lab.
All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically.
About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
No adherence issues with all of the validation slides that were run.
We have tried various types of slides recently as well, and the issue still prevails.

Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides.
I would welcome your suggestions and feedback.

Thank you so much!!!

Deanne Knutson
Supervisor
Anatomic Pathology

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