[Histonet] Opinion on dye on biopsies

[Bob Richmond]

John Garratt notes: >>Be aware that validation of IHC should be performed
if you are changing your processing protocol by adding a dye to the
reagents or to a pre-processed tissue. Be cautious!<<

I don't think safranin O would be a problem, but a good idea nonetheless!

On Mon, Jan 14, 2019 at 6:40 PM John Garratt <john.garratt at ciqc.ca> wrote:

> Be aware that validation of IHC should be performed if you are changing
> your processing protocol by adding a dye to the reagents or to a
> pre-processed tissue. Be cautious!
>
>
> John
>
>
>
> On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to
> > make sure they're recovered during embedding.
> >
> > I've had good results marking small specimens with the solution of
> safranin
> > O that's used in the microbiologists' Gram stain. Go to the micro lab and
> > ask for a small amount of it and try it.
> >
> > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence
> makes
> > it very difficult to do any kind of fluorescent stain on the sections.
> (It
> > also doesn't work as well as safranin, which isn't fluorescent.)
> >
> > Another necessary procedure at the gross desk: fill out a log sheet that
> > records the number of specimens you put into the cassette, and have that
> > log sheet in front of you when you embed. (I've had a lot of histotechs
> > flatly refuse to do this.)
> >
> > I like those little blue foam pads you put in the cassette and put the
> > small specimens on. I usually cut them in two before putting them in the
> > cassette.
> >
> > Bob Richmond
> > Samurai Pathologist
> > Maryville TN
> >
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>