[Histonet] Opinion on dye on biopsies
rsrichmond at gmail.com
Mon Jan 14 18:12:27 CST 2019
John Garratt notes: >>Be aware that validation of IHC should be performed
if you are changing your processing protocol by adding a dye to the
reagents or to a pre-processed tissue. Be cautious!<<
I don't think safranin O would be a problem, but a good idea nonetheless!
On Mon, Jan 14, 2019 at 6:40 PM John Garratt <john.garratt at ciqc.ca> wrote:
> Be aware that validation of IHC should be performed if you are changing
> your processing protocol by adding a dye to the reagents or to a
> pre-processed tissue. Be cautious!
> On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to
> > make sure they're recovered during embedding.
> > I've had good results marking small specimens with the solution of
> > O that's used in the microbiologists' Gram stain. Go to the micro lab and
> > ask for a small amount of it and try it.
> > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence
> > it very difficult to do any kind of fluorescent stain on the sections.
> > also doesn't work as well as safranin, which isn't fluorescent.)
> > Another necessary procedure at the gross desk: fill out a log sheet that
> > records the number of specimens you put into the cassette, and have that
> > log sheet in front of you when you embed. (I've had a lot of histotechs
> > flatly refuse to do this.)
> > I like those little blue foam pads you put in the cassette and put the
> > small specimens on. I usually cut them in two before putting them in the
> > cassette.
> > Bob Richmond
> > Samurai Pathologist
> > Maryville TN
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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