[Histonet] Eosin on processor for biopsies

[Cassie P. Davis]

Gareth,

reguarding the eosin biopsy post on the histo net. Things you might want to considered looking at, I cannot say for sure because I don't know your processing solutions/schedule:

Most Eosins are alcohol soluable, by putting the eosin in your formalin most of it will wash out during processing. Many labs put the Eosin in the last alcohol.

Hematoxylin is typically water soluable, if you put it in formailin processing usually removes water if the formailin is followed by alcohols.

I hope this helps,

Cassie


From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my processor.  My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting.  But, unfortunately, the esophagus tissues do not absorb the eosin much.  Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies.  I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain.
What are other labs doing to aid in making their small biopsies easier to see?  What are pros and cons to doing this, in your opinion?
Thanks!

--
*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364
928-248-5259
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