[Histonet] Opinion on dye on biopsies

John Garratt john.garratt at ciqc.ca
Mon Jan 14 17:40:50 CST 2019

Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious!


On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Gareth Davis asked about dyes to use to mark small GI biopsy specimens to
> make sure they're recovered during embedding.
> I've had good results marking small specimens with the solution of safranin
> O that's used in the microbiologists' Gram stain. Go to the micro lab and
> ask for a small amount of it and try it.
> Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes
> it very difficult to do any kind of fluorescent stain on the sections. (It
> also doesn't work as well as safranin, which isn't fluorescent.)
> Another necessary procedure at the gross desk: fill out a log sheet that
> records the number of specimens you put into the cassette, and have that
> log sheet in front of you when you embed. (I've had a lot of histotechs
> flatly refuse to do this.)
> I like those little blue foam pads you put in the cassette and put the
> small specimens on. I usually cut them in two before putting them in the
> cassette.
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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