[Histonet] IHC-H&E-IHC HELP...

[Greg Dobbin]

Hi Curt,
You can de-stain the hematoxylin counter stain and then do H&E and all
should be fine. Subsequent IHC is possible but obviously you would need a
different color chromogen to differentiate the new stain from the previous
one.

There may be a problem  getting the section stay on through a second IHC
procedure but maybe not. I would stain a control section for the desired Ab
using the retrieval method that was used initially and see how the control
looks. If the pathologist thinks the that this control is adequate for
interpretation, then restain the slides with the desired Ab and no
additional retrieval.

Cheers,
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0


*Everything in moderation...even moderation itself**!*