[Histonet] IHC Control Slide Longevity

[Jan Shivers]

Hi Sandy,

We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same
morning as staining; case material as well).

We've never had any problems with Synaptophysin; it's always worked very
well on unstained controls that are up to a year old (stored in the fridge).

Best regards,
Jan

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 at umn.edu

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On Thu, Feb 28, 2019 at 12:57 PM Sandra Cheasty via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello all,
>                 Do any of you have to cut any of you antibody controls
> "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We
> seldom do a Syanpt., and have found the fresh cut patient tissue has been
> positive while the not-so-fresh positive control slide is negative.
>                 Any comments are - as always, are appreciated.
> Cheers,
> Sandy
>
> Sandra J. Cheasty, HT (ASCP)
> Histology & Necropsy Supervisor
> UW-Madison, School of Veterinary Medicine
>
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