[Histonet] VIP issue

[Gudrun Lang]

Thank you for all the good hints and advices.

It has to be a reagens-issue, because the second instrument was also
concerned. 
But we had no similar problems for decades, although we use 96% as
cleaning-ethanol after ShellSol. 
We change the reagenses after 5 runs, once a week. 

We soon are forced to end using ShellSol, because the company stops the
production. We don't like to switch to xylene. 
Which xylen-substitutes are recommended for the VIP besides of Tissue-Clear
(Sakura)?

Best wishes
Gudrun 


-----Ursprüngliche Nachricht-----
Von: Gudrun Lang via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 11. Oktober 2018 18:58
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] VIP issue

Dear all!

I have a question for those, who are familiar with the VIPs from Sakura. 

Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.

What is the cause for such a case?  We have been using the organic solvent
(ShellSol) for decades as xylensubstitute. 

Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?

 

Thanks in advance

Gudrun Lang

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