[Histonet] VIP issue

[Laurie Redmond]

Either: 1) the ShellSol was over-used and could no longer clean/dissolve the paraffin so the remaining paraffin ended up in the alcohol  2) There was water in the cleaning alcohol (we always use 100% alcohol - do you always use 96%?  3) the cleaning ShellSol and cleaning alcohols were switched 4) there was a machine malfunction

Good Luck!
Laurie Redmond




-----Original Message-----
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu>
To: histonet <histonet at lists.utsouthwestern.edu>
Sent: Thu, Oct 11, 2018 10:58 am
Subject: [Histonet] VIP issue

Dear all!

I have a question for those, who are familiar with the VIPs from Sakura. 

Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.

What is the cause for such a case? We have been using the organic solvent
(ShellSol) for decades as xylensubstitute. 

Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?



Thanks in advance

Gudrun Lang

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