[Histonet] VIP issue
Laurie Redmond
boznpl at aol.com
Thu Oct 11 14:00:34 CDT 2018
Either: 1) the ShellSol was over-used and could no longer clean/dissolve the paraffin so the remaining paraffin ended up in the alcohol 2) There was water in the cleaning alcohol (we always use 100% alcohol - do you always use 96%? 3) the cleaning ShellSol and cleaning alcohols were switched 4) there was a machine malfunction
Good Luck!
Laurie Redmond
-----Original Message-----
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu>
To: histonet <histonet at lists.utsouthwestern.edu>
Sent: Thu, Oct 11, 2018 10:58 am
Subject: [Histonet] VIP issue
Dear all!
I have a question for those, who are familiar with the VIPs from Sakura.
Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.
What is the cause for such a case? We have been using the organic solvent
(ShellSol) for decades as xylensubstitute.
Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?
Thanks in advance
Gudrun Lang
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