[Histonet] VIP issue

[Joseph Saby]

 Gudrun-
Assuming this is a new issue with a recent reagent change, I suspect that the alcohol used for the clean cycle was not 100% (maybe 95%?).
The ability of your alcohol to hold the paraffin in solution is lost as the percentage of water increases.
I also expect your solvent was mostly saturated with paraffin.
Try changing your clean cycle reagents and see if the situation improves. I would do this before processing tissue again on this unit.

I hope this helps.
Joe Saby



On Thursday, October 11, 2018, 1:48:54 PM EDT, Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Dear all!

I have a question for those, who are familiar with the VIPs from Sakura. 

Last time we changed the reagenses the cleaning-ethanol (96%) was very milky
and even was full of many small particles. It was a paraffin-soup.

What is the cause for such a case?  We have been using the organic solvent
(ShellSol) for decades as xylensubstitute. 

Maybe the quality has suffered and the ability of solving paraffin has
decreased. But are there other explanations? Maybe a malfunction of the
instrument?

 

Thanks in advance

Gudrun Lang

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet