[Histonet] Oil Red O

Caroline Miller mills at 3scan.com
Wed Oct 3 11:17:57 CDT 2018


Hi Reuel,

In my experience this is the ORO crystallizing out on the section. I would
first filter your staining solution, then keep your staining times to as
short as you can. Also changing between 37 and room temperatures may be
useful too.

mills
ᐧ

On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> I was wondering if you could help me know why our Oil Red O have some
> black snowflakes on our fat tissue hours after staining or after 24 hours
> more snowlflakes precipitation occurs.  The staining was reference was from
> Lillie RD, Ashburn. Please note that our staining works well between two to
> three hours. Is there a reason fro this precipitation?
>
>
> Reuel
>
> TSRH
>
> Dallas, TX
>
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-- 
Caroline Miller (mills)
Director of Biology

www.3Scan.com
415 2187297


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